Cleaning Procedure For Biological Safety Cabinet

5 0 procedure 5 1 general cleaning procedure 5 1 1 ensure that the power supply of the biosafety cabinet is switched off.

Cleaning procedure for biological safety cabinet.

10 easy steps to cleaning a contaminated surface in the biosafety cabinet. The biohazard bag should never be kept outside the cabinet. Put the waste from the spill into a biohazard bag inside the cabinet. To begin apply disinfect to a towel and decontaminate and remove items from the area of the spill.

Once the cabinet is empty you should allow 20 30 minutes to thoroughly decontaminate all interior work surfaces making sure to clean the back and side of the cabinet and the interior of the glass. Cleaning a biosafety cabinet or bsc is an important function in terms of both containment and sterility. The interior surfaces should also be wiped with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet. Allow to air dry.

When work is completed all equipment and supplies from the bsc should be decontaminated and removed from the cabinet. This may take more or less time depending on the disinfectant and the microbiological agent used. Typically surface decontamination is adequate however there are instances such as extensive repair inside the cabinet envelope or hepa filter replacement that will require gas decontamination. Remember to wear appropriate ppe and leave the biosafety cabinet on during spill cleanup.

Hepa stands for high efficiency particulate air. Once the cabinet has been operational for 15 minutes disinfect all interior surfaces of the cabinet thoroughly by spraying with 70 ethanol. Afterward wipe the surface with a clean dry cloth to remove the moisture. μ microns in diameter with higher efficiency for both larger and.

How to clean a biological safety cabinet lafw hood. 5 1 2 dedust the biosafety cabinet daily externally with a clean dry cloth. Cleaning and disinfection 1. How to clean a biological safety cabinet lafw hood.

In addition periodic and thorough decontamination routines including disinfecting all removable parts and surfaces is recommended by the manufacturer and is a good laboratory practice to reduce wear on the equipment and provide greater safety to you your samples and the environment. Aseptic technique and daily decontamination will eliminate the majority of contaminants. Hepa filters are defined as air cleaning devices that have a proven minimum removal efficiency of 99 97 of particles in the air equal to 0 3. Biological safety cabinet sop bsc 01.

The biological safety officer will determine the type of decontamination necessary. 5 1 3 once in a week remove adhered dust by wet mopping using soap solution.